reduction of phosphate molybdenum vi to phosphate molybdenum by ascorbic acid

notes on the colorimetric determination of inorganic

in this paper that the most important factor in these methods is the temperature at which molybdenum blue color is developed the rate constants of acid hydrolysis of adenosine triphosphate glucose-i- phosphate fructose-1 6-diphosphate phosphoryl enol pyruvate and inorganic pyrophosphate at vari- ous temperatures

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the molybdenum blue reaction for the determination of

oct 9 the method is based on formation of 12molybdophosphoric acid h 3 pmo 12 o 40 by phosphate v ions with ammonium heptamolybdate- nh 4 6 mo 7 o 24 in an acidic environment reduction of this acid leads to formation of phosphomolybdenum blue that absorbs radiation of nm 27 28

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how to measure total phosphorus ascorbic acid method - national

page 10 ascorbic acid method orthophosphate reacts in acid medium with ammonium molybdate and potassium antimonyl tartrate to form phosphomolybdic acid this is reduced by ascorbic acid to form highly coloured molybdenum blue measured spectrophotometrically at nm arsenate chromium vi and

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molybdate/phosphate spectrophotometric analysis viper

aug 26 someone also mentioned being sure to do a dilute acid rinse of glassware to remove phosphate contamination from detergents? suggestions on the colored species is a molybdenum blue polyoxometalate formed from reduction of the heteropoly acid - phosphomolybdic acid of uncertain stoichiometry

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by the molybdenum blue method - europe pmc

by the molybdenum blue method by w i m reducing agent for phosphomolybdic acid and de- vised a rough test stopper and marked at levels corresponding to exactly 5 and 10 ml if the solution is acid or alkaline add phenol- phthalein and neutralize with n/10 naoh or n/10 hci 09-8 0 8 0-7 0-6 ' 0-5 0 4 -

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molybdate reduction to molybdenum blue by an antarctic bacterium

nov 9 metal ions such as cadmium chromium copper silver lead and mercury caused more than 95 inhibition of the molybdenum-reducing activity at 0 1 product that shows close similarity to mo-blue produced in the phosphate determination method using ascorbic acid as the reducing agent 25 48 49

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a modified molybdenum blue method for - semantic scholar

it involves the reaction of acid ammonium molybdate with pi ions to form phosphomolybdenum complexes which are reduced to molybdenum blue by ascorbic acid however it is known that the assay conditions used can cause partial hydrolysis of some labile organic phosphate po and condensed pi resulting in

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molybdenum blue - wikipedia

the following example describes the determination of phosphorus a sample containing the phosphate is mixed with an acid solution of movi for example ammonium molybdate to produce pmo 12o3 40 which has an α-keggin structure this anion is then reduced by for example ascorbic acid or sncl2 to form the blue

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a modified molybdenum blue method for - semantic scholar

it involves the reaction of acid ammonium molybdate with pi ions to form phosphomolybdenum complexes which are reduced to molybdenum blue by ascorbic acid however it is known that the assay conditions used can cause partial hydrolysis of some labile organic phosphate po and condensed pi resulting in

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a simplified method for inorganic phosphate determination and its

the method is sensitive easy economic and applicable for estimation of phosphate released in both enzymatic and nonenzymatic reactions a mixture of hydrazine sulfate and ascorbic acid was used as the reducing agent and the conditions for the development of the molybdenum blue color were optimized thus in the

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molybdenum blue - wikipedia

the following example describes the determination of phosphorus a sample containing the phosphate is mixed with an acid solution of movi for example ammonium molybdate to produce pmo 12o3 40 which has an α-keggin structure this anion is then reduced by for example ascorbic acid or sncl2 to form the blue

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spectrophotometric determination of phosphate in - springer link

b a reduced species of molybdophosphate molybdenum blue and of molyb- dophosphovanadate have been obtained in the presence of great number of reducing agents the most popular are fe ii 4 sn 5 ascorbic acid 6 and potassium antimonyl tartrate solution 7 c molybdophosphoric heteropoly acid reduced

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determination of aqueous phosphate by ascorbic acid reduction of

jan 1 determination of phosphate in selective extractions for soil iron oxides by the molybdenum blue method in an automated continuance flow injection system amir hass richard h loeppert michael g messina timothy d rogers communications in soil science and plant analysis 42 14

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a simplified method for inorganic phosphate determination and its

the method is sensitive easy economic and applicable for estimation of phosphate released in both enzymatic and nonenzymatic reactions a mixture of hydrazine sulfate and ascorbic acid was used as the reducing agent and the conditions for the development of the molybdenum blue color were optimized thus in the

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a single-solution method for the determination of soluble phosphate

of the reagents so far suggested for the reduction of phosphomolybdic acid to molybdenum blue in the determination of phosphate only stannous chloride atkins harvey metol burton riley and ascorbic acid greenfield kalber give colours of sufficient intensity to be used in the analysis of

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the molybdenum blue reaction for the determination of

oct 9 the method is based on formation of 12molybdophosphoric acid h 3 pmo 12 o 40 by phosphate v ions with ammonium heptamolybdate- nh 4 6 mo 7 o 24 in an acidic environment reduction of this acid leads to formation of phosphomolybdenum blue that absorbs radiation of nm 27 28

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determination of aqueous phosphate by ascorbic acid reduction of

jan 1 determination of phosphate in selective extractions for soil iron oxides by the molybdenum blue method in an automated continuance flow injection system amir hass richard h loeppert michael g messina timothy d rogers communications in soil science and plant analysis 42 14

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ascorbic acid method - shodhganga

appendix - vi ascorbic acid method the reagents used and the stepwise procedure of the ascorbic acid method used for phosphorus estimation in wastewaters is standard phosphate solution to obtain a calibration curve prepare a series reduced to intensely colored molybdenum blue by ascorbic acid

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mechanistic investigation of molybdenum blue method for

mechanistic investigation of molybdenum blue method for determination of phosphate stanley r crouch and journal of agricultural and food chemistry 62 6 - abstract full text html determination of aqueous phosphate by ascorbic acid reduction of phosphomolybdic acid theodore g towns

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the molybdenum blue reaction for the determination of - pearl

keywords molybdenum blue reaction orthophosphate dissolved reactive phosphate 23 2 2 mo vi speciation and 12-molybdophosphoric acid 12-mpa formation 4 2 3 redox chemistry of pmb 5 2 3 1 reduction of 12-mpa 6 2 3 2 spectral optimising its analytical application for the determination of phosphate

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the molybdenum blue reaction for the determination of - pearl

keywords molybdenum blue reaction orthophosphate dissolved reactive phosphate 23 2 2 mo vi speciation and 12-molybdophosphoric acid 12-mpa formation 4 2 3 redox chemistry of pmb 5 2 3 1 reduction of 12-mpa 6 2 3 2 spectral optimising its analytical application for the determination of phosphate

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microbiological reduction of hexavalent molybdenum to

in general microbes are able to survive the toxic effects of heavy metals by using a variety of mechanisms such as bioreduction to a less toxic state active pumping to remove heavy metals from the cytosolic environment bioprecipitation and sequestration to name a few molybdenum is one of the heavy metals that have

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how to measure total phosphorus ascorbic acid method - national

page 10 ascorbic acid method orthophosphate reacts in acid medium with ammonium molybdate and potassium antimonyl tartrate to form phosphomolybdic acid this is reduced by ascorbic acid to form highly coloured molybdenum blue measured spectrophotometrically at nm arsenate chromium vi and

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chapter 3 thiourea reduced phosphomolybdate ion - shodhganga

chapter 3 phosphate 63 sodium molybdate na2moo4 2h4o ammonium dimolybdate nh4 2mo2o7 and ammonium heptamolybdate nh4 6mo7o24 4h4o molybdenum vi ion does not exist in solution as free ion but exists as molybdate ion moo4 2- 6 under acidic conditions molybdate ion would combine and

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microbiological reduction of hexavalent molybdenum to

in general microbes are able to survive the toxic effects of heavy metals by using a variety of mechanisms such as bioreduction to a less toxic state active pumping to remove heavy metals from the cytosolic environment bioprecipitation and sequestration to name a few molybdenum is one of the heavy metals that have

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mechanistic investigation of molybdenum blue method for

mechanistic investigation of molybdenum blue method for determination of phosphate stanley r crouch and journal of agricultural and food chemistry 62 6 - abstract full text html determination of aqueous phosphate by ascorbic acid reduction of phosphomolybdic acid theodore g towns

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solved one common way to determine phosphorus in urine is to t

one common way to determine phosphorus in urine is to treat the sample after removing the protein with molybdenum vi and then reducing the resulting 12-molybdophosphate complex with ascorbic acid to give an intense blue-colored species called molybdenum blue the absorbance of molybdenum blue can be

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a modified molybdenum blue method for orthophosphate

it involves the reaction of acid ammonium molybdate with pi ions to form phosphomolybdenum complexes which are reduced to molybdenum blue by ascorbic acid however it is known that the assay conditions used can cause partial hydrolysis of some labile organic phosphate po and condensed pi resulting in

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method comparison table - emd millipore

this method reads orthophosphate orthophosphate reacts with ammonium molybdate and potassium antimonyl tartrate in an acid medium to form phosphomolybdic acid this is reduced to molybdenum blue by ascorbic acid if total phosphate is desired a digestion procedure using ammonium persulfate reagent must

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a modified molybdenum blue method for orthophosphate

it involves the reaction of acid ammonium molybdate with pi ions to form phosphomolybdenum complexes which are reduced to molybdenum blue by ascorbic acid however it is known that the assay conditions used can cause partial hydrolysis of some labile organic phosphate po and condensed pi resulting in

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