isolated by the flotation method following 1h of collagenase

a method for isolating smooth muscle cells from pig urinary bladder

urol res jan 21 1 49-53 a method for isolating smooth muscle cells from pig urinary bladder with low concentrations of collagenase and papain the relation between calcium concentration and isolated cell length schot r 1 van asselt e van mastrigt r author information 1 department of urology erasmus

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the acute phase protein serum amyloid a induces lipolysis and

apr 19 louis ethics committee and all subjects gave their written consent subjects on endocrine therapy or antihypertensive therapy and patients with malignant diseases were excluded mature adipocytes were isolated by the flotation method following 1h of collagenase roche digestion and several washes

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isolation of human hepatocytes by a two-step collagenase perfusion

sep 3 thus this method presents a modified version of a two-step collagenase perfusion procedure to isolate hepatocytes as described by seglen 1 previously however enzymatic methods have been shown to be superior as hepatocytes retain their structural integrity and function after isolation this method

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which is best method for isolation of islets from mouse pancreas?

if you end the isolation with less tubes picking them will be very fast i use histopaque- as a gradient wash 2 times and do 3-4 times self sedimentation usually after the final sedimentation i count them by handpicking from a 8 week old wt mouse the average is between - healthy islets after o/n culture the

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analysis and isolation of adipocytes by flow cytometry

cytometry free-floating fat cells are isolated from adipose tissue by digesting the tissue in a buffered collagenase solution following digestion undigested tissue fragments are removed by filtration and the adipocytes are separated from stromal/vascular cells by centrifugation and/or flotation there are numerous variations

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